Backbone terminal cyclization, max. kcat/Km: 1.3 x 10^6 M-1s-1
Specificity stringency: Prime-low, Nonprime-high
pH optimal: pH 6-6.5
Expression Platform:
E. coli
P. pastoris
Mutations:
-
Other Properties:
-
References:
[1]
Nguyen, G. K. T.; Wang, S.; Qiu, Y.; Hemu, X.; Lian, Y.; Tam, J. P. Butelase 1 is an Asx-specific ligase enabling peptide macrocyclization and synthesis. Nat. Chem. Biol. 2014, 10 (9), 732-738, Article. (Sequence, activity)DOI: 10.1038/nchembio.1586