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HaPAL1

Host Organism

Helianthus annuus L.

Type

PAL

Sequence

MACFSYRLICLLLVLMMVMALPNGAAAARRGSDYWDPFIRSPVDLEDDELGNGTRWALLVAGSKGYQSYRHQANVCHAYQILKRGGLKDENIVVFMYDDIATCDENPRPGTIIHHPEGGDVYAGVPKDYTGDAVTADNFFAVILGDKSSVKGGSGKVIDSKPDDRIFLYYTDHGAAGLLGMPEKPYVVANDFVEVLKKKHAMGTYKEMVIYLEACESGSIFEGLLPEDLNIYAITSTKPEEPSYIIYCPDMNPPPPPEYTTCLGDTFSVAWMEDSETHNLKKESLAQQINKVKERTSMFGTYANGSHVMEYGTKVIKPEKVYLYQGYNPETANLPANRIHFDKKMESVNQRDGDLIYLWQKYKRSSVSNRAEALKQMTETLRYMAHLDSSVDMIGVLLFGPQNGGSILRSSRGRGLPLVDDWDCLKSMTRLFEKHCGLLTEYGMKHMRAFANICNNLVEETEVEEAIIATCSGKNIGPYASLGAYSV

External Links

PDB ID: -
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I

Functions

  • Backbone terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] Hemu, X.; Chan, N. Y.; Liew, H. T.; Hu, S.; Zhang, X.; Serra, A.; Lescar, J.; Liu, C. F.; Tam, J. P. Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains. New Phytol. 2023, 238 (4), 1534-1545. (Sequence, activity) DOI: 10.1111/nph.18841