VyPAL5 - Peptide Ligase Database

Host Organism

Viola yedoensis Makino (a synonym of Viola philippica var. philippica)

Type

PAL

Sequence

MKLLAAGVILVSLLALSGTVAVAVAGGLDVDPLRLPSEAAKFFHNDNSTNDDDSIGTTWAVLIAGSKGYHNYRHQADVCHMYQILRKGGVKDENIIVFMYDDIAYNESNPFPGIIINKPGGENVYKGVPKDYTGEDINNVNFLAAILGNKSAIIGGSGKVLDTSPNDHIFIYYADHGAPGKIGMPSKPYLYADDLVDTLKQKAATGTYKSMVFYVEACNAGSMFEGLLPEGTNIYAMAASNSTEGSWITYCPGTPDFPPEFDVCLGDLWSITFLEDCDAHNLRTETVHQQFELVKKKIAYASTVSQYGDIPISKDSLSVYMGTDPANDNRTFVDENSLRPPLKVIHQRDAYLYHLWYKYQNTPEGSSKKIEAQKQLLEMMSHRAHVDNSITLIGKLLFGMDKASKMLNSVRPAGQPLVDDWQCLKTMIRTFERHCGSLSEYGMKHTLSFANMCNAGIRKEQLAEAAAQACVTFPSNSYSSLAEGFSA

External Links

PDB ID: -

EC: 3.4.22.34

MEROPS: C13 legumain

UniProt ID: -

NCBI accession: -

Substrate Specificity

P1'

P2'

N/D

L/I/F

Functions

Backbone terminal cyclization
Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

E. coli

Mutations:

Other Properties:

References:

[1] Hemu, X.; El Sahili, A.; Hu, S.; Wong, K.; Chen, Y.; Wong, Y. H.; Zhang, X.; Serra, A.; Goh, B. C.; Darwis, D. A.; et al. Structural determinants for peptide-bond formation by asparaginyl ligases. Proc. Natl. Acad. Sci. U.S.A. 2019, 116 (24), 11737-11746. (Sequence) DOI: 10.1073/pnas.1818568116
[2] Hemu, X.; Chan, N. Y.; Liew, H. T.; Hu, S.; Zhang, X.; Serra, A.; Lescar, J.; Liu, C. F.; Tam, J. P. Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains. New Phytol. 2023, 238 (4), 1534-1545. (Activity) DOI: 10.1111/nph.18847