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HeAEP3

Host Organism

Hybanthus enneaspermus (L.) F. V. Muell. (a synonym of Pigea enneasperma)

Type

PAL

Sequence

MKLLVPGVLLLFLLALSGIAAGRPDDFLRLPSEAAKSFLHNDDDSVGTRWAVLIAGSKGWQNYRHQADVCHAYQILKKGGLKDENIIVFMYDDIAYNESNPRPGIVINKPKGEDVYKGVPKDYTGENVNAVNFLAVLLANRSALTGGSGKVLDSGPNDRIFIYYTDHGAPVTIGMPSKPYLVAKDLVDTLKKKHAAGTYKSMVFYIESCESGSMFDGLLPEDANIYGMTATNSTEGSWVTYCPGQTDDYPEDDEYDVCFGDLWSVAWLEDCDAHNLRTETLDQQYEVVKKKIEYAHIPAQYGNVSLAKDSLFVYMGTDPANDNKTFVEENTLRRPLKAVHSRDADLLHFWHKYHKAPEGTSRKIDAQKQLVEVLSHRTHVDNSIKLVGELLFGVGKASEVLNTIRPAGQPLVDDWDCLKTMVRTFETHCGSLSEYGMKHMRSFANMCNAGVQKEQMAVAAGQACVTFPSNPWSSLDEGFSV

External Links

PDB ID: -
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I

Functions

  • Backbone terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] Jackson, M. A.; Gilding, E. K.; Shafee, T.; Harris, K. S.; Kaas, Q.; Poon, S.; Yap, K.; Jia, H.; Guarino, R.; Chan, L. Y.; et al. Molecular Basis for the Production of Cyclic Peptides by Plant Asparaginyl Endopeptidases. Nat Commun 2018, 9 (1), 2411. (Sequence) DOI: 10.1038/s41467-018-04669-9
  2. [2] Hemu, X.; Chan, N. Y.; Liew, H. T.; Hu, S.; Zhang, X.; Serra, A.; Lescar, J.; Liu, C. F.; Tam, J. P. Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains. New Phytol. 2023, 238 (4), 1534-1545. (Classification, Activity) DOI: 10.1111/nph.18848