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OaAEP1b

Host Organism

Oldenlandia affinis (Roem. & Schult.) DC.

Type

PAL

Sequence

MVRYLAGAVLLLVVLSVAAAVSGARDGDYLHLPSEVSRFFRPQETNDDHGEDSVGTRWAVLIAGSKGYANYRHQAGVCHAYQILKRGGLKDENIVVFMYDDIAYNESNPRPGVIINSPHGSDVYAGVPKDYTGEEVNAKNFLAAILGNKSAITGGSGKVVDSGPNDHIFIYYTDHGAAGVIGMPSKPYLYADELNDALKKKHASGTYKSLVFYLEACESGSMFEGILPEDLNIYALTSTNTTESSWCYYCPAQENPPPPEYNVCLGDLFSVAWLEDSDVQNSWYETLNQQYHHVDKRISHASHATQYGNLKLGEEGLFVYMGSNPANDNYTSLDGNALTPSSIVVNQRDADLLHLWEKFRKAPEGSARKEEAQTQIFKAMSHRVHIDSSIKLIGKLLFGIEKCTEILNAVRPAGQPLVDDWACLRSLVGTFETHCGSLSEYGMRHTRTIANICNAGISEEQMAEAASQACASIP

External Links

PDB ID: 5H0I
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: A0A0N9JZ32
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization
  • Side-chain to C-terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

  • E. coli
  • N. benthamiana

Mutations:

  • Fast kinetics: C247A

Other Properties:

-

References:

  1. [1] Harris, K. S.; Durek, T.; Kaas, Q.; Poth, A. G.; Gilding, E. K.; Conlan, B. F.; Saska, I.; Daly, N. L.; van der Weerden, N. L.; Craik, D. J.; et al. Efficient Backbone Cyclization of Linear Peptides by a Recombinant Asparaginyl Endopeptidase. Nat Commun 2015, 6, 10199. (Sequence, activity) DOI: 10.1038/ncomms10199