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ConPAL3

Host Organism

Artificial

Type

PAL

Sequence

MGSSHHHHHHSSGLVPRGSHMARDLGGDVLRLPSEAARFFRGNSNDDDSVGTRWAVLIAGSNGYWNYRHQADVCHAYQLLRKGGLKDENIIVFMYDDIAYNEENPRPGVIINSPHGEDVYKGVPKDYTGEDVTVNNFFAVILGNKTALTGGSGKVVDSGPNDHIFIYYSDHGAPGVLGMPSKPYLYADDLIDVLKKKHASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWVTYCPGEYPSPPPEYETCLGDLYSVAWMEDSDIHNLRTETLHQQYELVKRRTAGYASHVMQYGDLGLSKDNLFLYMGTNPANDNYTFVDENSLRSPLKAVNQRDADLVHFWEKYRKAPEGSPRKVEAQKQFLEAMSHRMHIDNSVKLIGKLLFGIEKGPEVLNAVRPAGQPLVDDWDCLKSMVRTFETHCGSLSQYGMKHMRSFANICNAGIQKEQMAEASAQACVSIPLEHHHHHH

External Links

PDB ID: -
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I/F

Functions

  • Backbone terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] Hemu, X.; Zhang, X.; Chang, H. Y.; Poh, J. E.; Tam, J. P. Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation. J Biol Chem 2023, 299 (3), 102997. (Sequence, activity, engineering) DOI: 10.1016/j.jbc.2023.102997