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Subtiligase

Host Organism

Bacillus amyloliquefaciens

Type

Subtiligase

Sequence

NAN

External Links

PDB ID: -
EC: 3.4.21.62
MEROPS: S8 subtilase
UniProt ID: P00782
NCBI accession: -

Substrate Specificity

P2
P1
P1'
A/S/G
L/F/A/V
Ester/thioester

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-low, only stringent to the C-terminal active ester/thioester served as a leaving group

Expression Platform:

  • E. coli

Mutations:

  • Protease-to-ligase conversion: S221C/P225A

Other Properties:

-

References:

-