Specificity stringency: Prime-low, Nonprime-low, only stringent to the C-terminal active ester/thioester served as a leaving group
Expression Platform:
E. coli
Mutations:
Improve stability, Calcium-indepenency: a deleted calcium binding domain (deletion of 9 amino acids, number 75 to 83) and additional 18 stabilizing mutations, including one disulfide bridge
Other Properties:
-
References:
[1]
(Engineering, Sequence) Toplak, A.; Nuijens, T.; Quaedflieg, P. J. L. M.; Wu, B.; Janssen, D. B. Peptiligase, an Enzyme for Efficient Chemoenzymatic Peptide Synthesis and Cyclization in Water. Advanced Synthesis & Catalysis 2016, 358 (13), 2140-2147.
DOI: https://doi.org/10.1002/adsc.201600017