Specificity stringency: Prime-low, Nonprime-low, only stringent to the C-terminal active ester/thioester served as a leaving group
Expression Platform:
B. subtilis
Mutations:
Tailor substrate specificity
Other Properties:
-
References:
[1]
(Engineering) Schmidt, M.; Toplak, A.; Rozeboom, H. J.; Wijma, H. J.; Quaedflieg, P.; van Maarseveen, J. H.; Janssen, D. B.; Nuijens, T. Design of a substrate-tailored peptiligase variant for the efficient synthesis of thymosin-α1. Org Biomol Chem 2018, 16 (4), 609-618.
DOI: 10.1039/c7ob02812a