Back to All Enzymes

PatG

Host Organism

Prochloron didemni

Type

Subtilisin-like ligase

Sequence

GSHMNRDILRTLSLKGDHNIRVAILDGPVDIAHPCFQGADLTVLPTLAPTAARSDGFMSAHGTHVASIIFGQPETSVPGIAPQCRGLIVPIFSDDRRRITQLDLARGIERAVNAGAHIINISGGELTDFGEADGWLENAVSLCRQNNVLLVAAAGNNGCDCLHVPAALPAVLAVGAMDDHGHPLDFSNWGSTYEQQGILAPGEDILGAKPGGGTERLSGTSFATPIVSGVAALLLSEQVRRGETPDPQKVRQLLLQSALPCDDDAPEQARRCLAGRLNVSGAFTLLKGGNMSEELATASFPSVEAS

External Links

PDB ID: 4AKS
EC: -
MEROPS: S8 subtilase
UniProt ID: -
NCBI: -

Substrate Specificity

P1
P1'
P2'
P3'
P4'
P5'
Pro/Thiazole/Thiazoline/Oxazoline
A
Y
D
G
E

Functions

  • Backbone terminal cyclization of short peptides[2]
  • Specificity stringency: Prime-high, Nonprime-low[2]

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Discovery) Schmidt, E. W.; Nelson, J. T.; Rasko, D. A.; Sudek, S.; Eisen, J. A.; Haygood, M. G.; Ravel, J. Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni, the cyanobacterial symbiont of Lissoclinum patella. Proc. Natl. Acad. Sci. U.S.A. 2005, 102 (20), 7315-7320. DOI: 10.1073/pnas.0501424102
  2. [2] (Activity) Lee, J.; McIntosh, J.; Hathaway, B. J.; Schmidt, E. W. Using Marine Natural Products to Discover a Protease that Catalyzes Peptide Macrocyclization of Diverse Substrates. J. Am. Chem. Soc. 2009, 131 (6), 2122-2124. DOI: 10.1021/ja8092168
  3. [3] (Specificity) McIntosh, J. A.; Robertson, C. R.; Agarwal, V.; Nair, S. K.; Bulaj, G. W.; Schmidt, E. W. Circular Logic: Nonribosomal Peptide-like Macrocyclization with a Ribosomal Peptide Catalyst. J. Am. Chem. Soc. 2010, 132 (44), 15499-15501. DOI: 10.1021/ja1067806
  4. [4] (Structure) Agarwal, V.; Pierce, E.; McIntosh, J.; Schmidt, E. W.; Nair, S. K. Structures of cyanobactin maturation enzymes define a family of transamidating proteases. Chem Biol 2012, 19 (11), 1411-1422. DOI: 10.1016/j.chembiol.2012.09.012
  5. [5] (Mechanism) Koehnke, J.; Bent, A.; Houssen, W. E.; Zollman, D.; Morawitz, F.; Shirran, S.; Vendome, J.; Nneoyiegbe, A. F.; Trembleau, L.; Botting, C. H.; et al. The mechanism of patellamide macrocyclization revealed by the characterization of the PatG macrocyclase domain. Nat. Struct. Mol. Biol. 2012, 19 (8), 767-772. DOI: 10.1038/nsmb.2340