PatGmac - Peptide Ligase Database

Host Organism

Prochloron didemni

Type

Subtilisin-like ligase

Sequence

GEDEIESAGVSASEVESSATKQKVALHPHDLDERIPGLADLHNQTLGDPQITIVIIDGDPDYTLSCFEGAEVSKVFPYWHEPAEPITPEDYAAFQSIRDQGLKGKEKEEALEAVIPDTKDRIVLNDHACHVTSTIVGQEHSPVFGIAPNCRVINMPQDAVIRGNYDDVMSPLNLARAIDLALELGANIIHCAFCRPTQTSEGEEILVQAIKKCQDNNVLIVSPTGNNSNESWCLPAVLPGTLAVGAAKVDGTPCHFSNWGGNNTKEGILAPGEEILGAQPCTEEPVRLTGTSMAAPVMTGISALLMSLQVQQGKPVDAEAVRTALLKTAIPCDPEVVEEPERCLRGFVNIPGAMKVLFGQ

External Links

PDB ID: 4AKS

EC: -

MEROPS: S8 subtilase

UniProt ID: -

NCBI: -

Substrate Specificity

P1'

P2'

P3'

P4'

P5'

P/Thiazolidine

Functions

Backbone terminal cyclization of short peptides[2]
Specificity stringency: Prime-high, Nonprime-low[2]

Expression Platform:

E. coli

Mutations:

Other Properties:

References:

[1] (Engineering) Koehnke, J.; Bent, A.; Houssen, W. E.; Zollman, D.; Morawitz, F.; Shirran, S.; Vendome, J.; Nneoyiegbe, A. F.; Trembleau, L.; Botting, C. H.; et al. The mechanism of patellamide macrocyclization revealed by the characterization of the PatG macrocyclase domain. Nature Structural & Molecular Biology 2012, 19 (8), 767-772. DOI: 10.1038/nsmb.2340