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Sortase A

Host Organism

Staphylococcus aureus

Type

Sortase

Sequence

MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

External Links

PDB ID: 1IJA, 1T2O, 1T2P, 1T2W, 2KID, 6R1V, 7S54
EC: 3.4.22.70
MEROPS: C60 sortase
UniProt ID: Q9S446
NCBI: -

Substrate Specificity

P4
P3
P2
P1
P1'
L
P
X
T
G

Functions

  • Backbone terminal ligation
  • Backbone cyclization
  • Terminal to amine ligaiton
  • Specificity strigency: Prime-high, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Discovery) Mazmanian, S. K.; Liu, G.; Ton-That, H.; Schneewind, O. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science 1999, 285 (5428), 760-763. DOI: 10.1126/science.285.5428.760
  2. [2] (Function) Ton-That, H.; Mazmanian, S. K.; Alksne, L.; Schneewind, O. Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. J. Biol. Chem. 2002, 277 (9), 7447-7452. DOI: 10.1074/jbc.M109945200