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ESrtA

Host Organism

Staphylococcus aureus

Type

Sequence

MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKPTAVGVLDEQKGKDKQLTLITCDDYNEETGVWETRKIFVATEVK

External Links

PDB ID: -
EC: 3.4.22.70
MEROPS: C60 sortase
UniProt ID: -
NCBI: -

Substrate Specificity

P4
P3
P2
P1
P1'
L
P
X
T
G

Functions

  • Backbone terminal ligation
  • Backbone cyclization
  • Terminal to amine ligaiton
  • Specificity strigency: Prime-high, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

  • Fast kinetics: P94R/D160N/D165A/K190E/K196T

Other Properties:

-

References:

  1. [1] (Engineering, evolution) Chen, I.; Dorr, B. M.; Liu, D. R. A general strategy for the evolution of bond-forming enzymes using yeast display. Proceedings of the National Academy of Sciences 2011, 108 (28), 11399-11404. DOI: 10.1073/pnas.1101046108
  2. [2] (Application-Shift reaction equilibrium by electrostatic-difference of substrates) Wang, C.; Desmet, R.; Snella, B.; Vicogne, J.; Melnyk, O.; Agouridas, V. Leveraging Sortase A Electrostatics for Powerful Transpeptidation Reactions. Angew Chem Int Ed Engl 2025, e202507236. DOI: 10.1002/anie.202507236