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ESrtA(2A-9)

Host Organism

Staphylococcus aureus

Type

Sequence

MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVCFHDENESLDDQNISIAGHTFIDRPNYQFTNLKAAKPGSMVYFKVGNETRIYKMTSIRKVHPNAVGVLDEQEGKDKQLTLVTCDDYNEETGVWESRKIFVATEVK

External Links

PDB ID: -
EC: 3.4.22.70
MEROPS: C60 sortase
UniProt ID: -
NCBI: -

Substrate Specificity

P4
P3
P2
P1
P1'
L
A
X
T
G

Functions

  • Backbone terminal ligation
  • Backbone cyclization
  • Terminal to amine ligaiton
  • Specificity strigency: Prime-high, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

  • Change substrate specificity to LAXTG:S102C A104H E105D K138P K152I N160K K162H T164N K173E I182V T196S (based on eSrtA)

Other Properties:

-

References:

  1. [1] (Engineering, Change specificity scope) Dorr, B. M.; Ham, H. O.; An, C. H.; Chaikof, E. L.; Liu, D. R. Reprogramming the specificity of sortase enzymes. Proc. Natl. Acad. Sci. U.S.A. 2014, 111 (37), 13343-13348. DOI: 10.1073/pnas.1411179111