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SrtA7M

Host Organism

Staphylococcus aureus

Type

Sortase

Sequence

MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVSFAKENASLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKPTAVGVLDEQKGKDKQLTLITCDDYNEETGVWETRKIFVATEVK

External Links

PDB ID: -
EC: 3.4.22.70
MEROPS: C60 sortase
UniProt ID: -
NCBI: -

Substrate Specificity

P4
P3
P2
P1
P1'
P2'
L
P
X
T
G
G

Functions

  • Backbone terminal ligation
  • Backbone cyclization
  • Terminal to amine ligaiton
  • Specificity strigency: Prime-high, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

  • Fast kinetics and Ca2+-independent: P94R/E105K/E108A/D160N/D165A/K190E/K196T

Other Properties:

-

References:

  1. [1] (Engineering, Application) Wu, Q.; Ploegh, H. L.; Truttmann, M. C. Hepta-Mutant Staphylococcus aureus Sortase A (SrtA7m) as a Tool for in Vivo Protein Labeling in Caenorhabditis elegans. ACS Chem Biol 2017, 12 (3), 664-673. DOI: 10.1021/acschembio.6b00998