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GmPOPB

Host Organism

Galerina marginata

Type

Sequence

NAN

External Links

PDB ID: 5N4B
EC: -
UniProt ID: -
NCBI accession: -

Substrate Specificity

-

Functions

  • Backbone terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

  • S. cerevisiae,E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] Luo, H.; Hong, S. Y.; Sgambelluri, R. M.; Angelos, E.; Li, X.; Walton, J. D. Peptide Macrocyclization Catalyzed by a Prolyl Oligopeptidase Involved in alpha-Amanitin Biosynthesis. Chem. Biol. 2014, 21 (12), 1610-1617. (Discovery, Recombinant expression) DOI: 10.1016/j.chembiol.2014.10.015
  2. [2] Czekster, C. M.; Ludewig, H.; McMahon, S. A.; Naismith, J. H. Characterization of a dual function macrocyclase enables design and use of efficient macrocyclization substrates. Nat Commun 2017, 8 (1), 1045. (Structure, Mechanism) DOI: 10.1038/s41467-017-00862-4