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SrtAβ

Host Organism

Staphylococcus aureus

Type

Sortase

Sequence

MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYLEIPDADIKEPVYPGPATREQLDRGVCFVDEDESLDDQNISIIGHTALLRPHYQFTNLRAAKLDSIVYFTVGNETRRYKITIICNVRPTNVGVLAEHEGKDRQLTLATCDDYNYETGVWESSKIFVATEVR

External Links

PDB ID: -
EC: 3.4.22.70
MEROPS: C60 sortase
UniProt ID: -
NCBI: -

Substrate Specificity

P4
P3
P2
P1
P1'
L
M
V
G
G

Functions

  • Covalent biotinylation of Aβ

Expression Platform:

  • S. cerevisiae
  • E. coli BL21(DE3)

Mutations:

  • Tailered substrate spcecificity to recognize LMVGG: N98D/I76L/S102C/A104V/E105D/N107D/A118I/F122A/I123L/D124L/N127H/K134R/K138L/G139D/M141I/K145T/K152R/M155I/S157I/R159C/K162R/Q172H/K173E/K177R/I182A/E189Y/T196S/R197S/K206R(compared with wtSrtA)

Other Properties:

-

References:

  1. [1] (Directed evolution, Modified substrate specificity) Podracky, C. J.; An, C.; DeSousa, A.; Dorr, B. M.; Walsh, D. M.; Liu, D. R. Laboratory evolution of a sortase enzyme that modifies amyloid-beta protein. Nat. Chem. Biol. 2021, 17 (3), 317-325. DOI: 10.1038/s41589-020-00706-1