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Sortase D

Host Organism

Bacillus anthracis

Type

Sequence

MILMAIGLLMGSYYAVEWYKGKSSAQELTNEEIKSFKNIQHNQLPYETLVTSQVPSSQTEHKEGEKVAMLNIPKLKKKFSIYWGADDATLKKGVGMFVSDVTTTPSGGGHTVLSGHRDTVFTDLGQLKEKDTLVLEYDNKTYTYEIQKIWITHADDRTVIIKKEEPILTLTTCYPFDYIGDAPDRYIIEAKLTGSYSK

External Links

PDB ID: 2LN7
EC: -
MEROPS: -
UniProt ID: -
NCBI: -

Substrate Specificity

P4
P3
P2
P1
P1'
P2'
L
P
N
T
A
G

Functions

-

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Discovery) Budzik, J. M.; Oh, S. Y.; Schneewind, O. Cell wall anchor structure of BcpA pili in Bacillus anthracis. J Biol Chem 2008, 283 (52), 36676-36686. DOI: 10.1074/jbc.M806796200
  2. [2] (Activity) (function) Budzik, J. M.; Oh, S. Y.; Schneewind, O. Sortase D forms the covalent bond that links BcpB to the tip of Bacillus cereus pili. J Biol Chem 2009, 284 (19), 12989-12997. DOI: 10.1074/jbc.M900927200
  3. [3] (Structure) Robson, S. A.; Jacobitz, A. W.; Phillips, M. L.; Clubb, R. T. Solution Structure of the Sortase Required for Efficient Production of Infectious Bacillus anthracis Spores. Biochemistry 2012, 51 (40), 7953-7963. DOI: 10.1021/bi300867t