Back to All Enzymes

Connectase

Host Organism

Methanosarcina mazei

Type

Connectase

Sequence

MSLIICYYGKNGAVIGGDRRQIFFRGSEENRKILEEKLYSGEIKSEEELYKLAEKLNIKIIIEDDREKVRKISDSVVCGEVRSLGIDAKRRRVYATKGKCAIVDILNDTVTNQTIKEGFGIVVLGNRFLKKKAEEELKRTAKLFPMMPIQQIEDAIKEIFEKLKWHPTVSKEYDIYSVNKYEKNFEEVIKKDIESLFKYREQLRKQLIDFGKVMSIVNKIVKNGEIGVIKDGKLHLYDDYIAIDKIDPNPKVFKVVDVEGNFKDGDIVVIENGDMKIKGTNEKVTTKYIIIHK

External Links

PDB ID: -
EC: -
MEROPS: -
UniProt ID: -
NCBI: -

Substrate Specificity

P2
P1
P1'
P2'
P3'
K
D
P
G
A

Functions

  • Intermolecular backbone terminal ligation
  • Specificity stringency: Prime-high, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Discovery, Sequence, Substrate specificity) Fuchs, A. C. D.; Ammelburg, M.; Martin, J.; Schmitz, R. A.; Hartmann, M. D.; Lupas, A. N. Archaeal Connectase is a specific and efficient protein ligase related to proteasome beta subunits. Proc Natl Acad Sci U S A 2021, 118 (11). DOI: 10.1073/pnas.2017871118
  2. [2] (Application) Fuchs, A. C. D. Specific, sensitive and quantitative protein detection by in-gel fluorescence. Nat Commun 2023, 14 (1), 2505. DOI: 10.1038/s41467-023-38147-8