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SurE

Host Organism

Streptomyces albidoflavus

Type

PBP-like cyclase

Sequence

MGAEGAERDAVGALFEELVREHRVTGAQLSVYRDGALSEYATGLASVRTGEPVTPRTGFPFGSVTKFLTAELVMQFVCDGDLDLDDPLAGLLPDLGRAAGPPLGTATVRQLLSHTAGVVDSIEYDEMRGPSYRRFAAACARQPALFPPGLAFSYSNTGYCLLGAVIEAASGMDWWTAMDSCLLRPLGIEPAFLHDPRPGQGGAARPVAEGHALRAGGERAEHVDHMASLSLAAAGGLVGSATDLVTAARPHLADRKTFAQHDLLPEDAVLAMRTCVPDAEPFGLADGWGLGLMRHGTGDGAWYGHDGAVGGASCNLRIHPDRSLALALTANSTAGPKLWEALVARLPEAGLDVGHYALPVPDSAPLAPDAGHLGTYANGDLELMVTHDAAGDLFLTRESYSDYRLSLHEDDLFVARSGEPGALPITGRFVREHPAGPVALLQYGGRAMHRL

External Links

PDB ID: -
EC: -
MEROPS: -
UniProt ID: -
NCBI: BBZ90014.1

Substrate Specificity

P1
P1'
P1''
dAA
ester
Hydrophobic

Functions

  • Backbone terminal cyclization of short peptides with acylated C-terminus
  • Specificity stringency: Prime-high, Nonprime-low[2]

Expression Platform:

  • E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] (Discovery, Sequence) Kuranaga, T.; Matsuda, K.; Sano, A.; Kobayashi, M.; Ninomiya, A.; Takada, K.; Matsunaga, S.; Wakimoto, T. Total Synthesis of the Nonribosomal Peptide Surugamide B and Identification of a New Offloading Cyclase Family. Angew. Chem. Int. Ed. 2018, 57 (30), 9447-9451. DOI: 10.1002/anie.201805541
  2. [2] (Discovery, Activity) Zhou, Y.; Lin, X.; Xu, C.; Shen, Y.; Wang, S. P.; Liao, H.; Li, L.; Deng, H.; Lin, H. W. Investigation of Penicillin Binding Protein (PBP)-like Peptide Cyclase and Hydrolase in Surugamide Non-ribosomal Peptide Biosynthesis. Cell Chem Biol 2019, 26 (5), 737-744 e734. DOI: 10.1016/j.chembiol.2019.02.010
  3. [3] (Application) Matsuda, K.; Kobayashi, M.; Kuranaga, T.; Takada, K.; Ikeda, H.; Matsunaga, S.; Wakimoto, T. SurE is a trans-acting thioesterase cyclizing two distinct non-ribosomal peptides. Organic & Biomolecular Chemistry 2019, 17 (5), 1058-1061. DOI: 10.1039/c8ob02867b
  4. [4] ()
  5. [5] (Substrate specificity, Activity, Engineering) Kobayashi, M.; Fujita, K.; Matsuda, K.; Wakimoto, T. Streamlined Chemoenzymatic Synthesis of Cyclic Peptides by Non-ribosomal Peptide Cyclases. J Am Chem Soc 2023, 145 (6), 3270-3275. DOI: 10.1021/jacs.2c11082