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Trypsiligase

Host Organism

Bos taurus

Type

Trypsiligase

Sequence

IVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYESGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGHTKSSGTSHPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKKSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN

External Links

PDB ID: -

EC: 3.4.21.4

MEROPS: S1 Trypsin

UniProt ID: P00760

NCBI: -

Substrate Specificity

P2

P1

P1'

Y

R

H

Functions

Intermolecular backbone terminal ligation
Backbone terminal cyclization
Specificity stringency: tripeptide recognition motif, Prime-high, Nonprime-high

Expression Platform:

E. coli

Mutations:

Protease-to-ligase conversion: K60E/N143H/E151H/D189K

Other Properties:

-

References:

[1] (Engineering) Liebscher, S.; Schopfel, M.; Aumuller, T.; Sharkhuukhen, A.; Pech, A.; Hoss, E.; Parthier, C.; Jahreis, G.; Stubbs, M. T.; Bordusa, F. N-terminal protein modification by substrate-activated reverse proteolysis. Angew. Chem. 2014, 53 (11), 3024-3028, Research Support, Non-U.S. Gov't. DOI: 10.1002/anie.201307736