Back to All Enzymes

Intein-Mxe GyrA

Host Organism

Mycobacterium xenopi

Type

Sequence

ASITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYAVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAKAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHN

External Links

PDB ID: 1AM2
EC: -
MEROPS: -
UniProt ID: -
NCBI: -

Substrate Specificity

P1'
Cys/Thr/Ser

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization
  • C-terminal thioester formation
  • Specificity stringency: Prime-low, Nonprime-low, only stringent to the P1"-Cys

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Structure) Klabunde, T.; Sharma, S.; Telenti, A.; Jacobs, W. R., Jr.; Sacchettini, J. C. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol 1998, 5 (1), 31-36. DOI: 10.1038/nsb0198-31