Back to All Enzymes

Npu DnaE split intein

Host Organism

Nostoc punctiforme

Type

Sequence

MSFVPLHIHSDYSLLDGASQLPELVDQAIALGMKAIALTDHGVMYGAVELIKICRSQNIKPIIGNEMYVINGDIEKQERRPKYHQVVLAKNTKGYKNLVKLTTISHLQGVQGKGIFSRPCINKDLLKQYHEGLIVTSACLGGEVPQAILSNRPDAARKVAQWYKDVFGDDYYLEIQDHGSQEDRIVNVEIVKIARELGIKIIATNDSHFISCFDVEAHDALLCIQTGKLIIEDKRMRYSGTEYLKSGEEMRQLFRDHLPDDVISEAVATTEEVADKVEPYHIMGEPQIPTPPIPSGHTADTYAEDVAWNGLLERLNRKSRQEVDSVYKERLEYELKMIQQMGFSKYFLVVWDYIKFARDNNIPVGPGRGSAAGSLVAYAMRITNIDPVHHGLLFERFLNPERKSMPDIDTDFCIEQRDKVIEYVTEKYGADRVAQIITFNRLTSKAVLKDVARVLNIPYGEADKMAKLIPVVRGKPTKLKVMVSDKTPEPEFKEKYDKEPHVRHWLDMAMRIEGTNKTFGVHAAGVVISDEPLDEIVPLQKNNDGSVITQYFMEDLESMGLLKMDFLGLRNLTLIQKTVDLIQETRGYRVDPDEIPRQERKAQKILAKGEHSSLPKDVQKTYELLEAGELEGIFQLESSGMRQIVRDLKPSNIEDISSILALYRPGPLDAGLIPKFINRKHGRENIDYQHTVLEPILDETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKVSEMQKQREKFVDGAAKNGVPKKVADELFEQMLKFAEYCLSYETEILTVEYGLLPIGKIVEKRIECTVYSVDNNGNIYTQPVAQWHDRGEQEVFEYCLEDGSLIRATKDHKFMTVDGQMLPIDEIFERELDLMRVDNLPN

External Links

PDB ID: 4QFQ
EC: -
MEROPS: -
UniProt ID: -
NCBI: -

Substrate Specificity

P1'
Cys/Thr/Ser

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization
  • C-terminal thioester formation
  • Specificity stringency: Prime-low, Nonprime-low, only stringent to the P1"-Cys

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Discovery) Zettler, J.; Schütz, V.; Mootz, H. D. The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction. FEBS Letters 2009, 583 (5), 909-914. DOI: https://doi.org/10.1016/j.febslet.2009.02.003