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Ssp DnaE split intein

Host Organism

Synechocystis sp.

Type

Sequence

MSFVGLHIHSDYSLLDGASQLPALIDRAIELGMPAIALTDHGVMYGAVELLKVCRGKPIKPIIGNEMYVINGDIEINKRHRRFHQVVLAKNNQGYRNLVKLTTISNLKGIQGSGIFARPCINKELLEQYKEGLIVTSACLGGEVPQAILKGDLDHARQVAKWYKNLFGDDYYLEIQDHGSVEDRLVNINLVKIAQELDIKIVATNDSHFISCNDVEAHDALLCIQTGKLITEEKRLRYSGTEYLKSAEEMHLLFRDHLPDDIIETAIANTLEVADKVEAYSILGEPRIPHYPIPPNHTPETYVEDIAWDGLLERLKCRERADITPVYKERLEYELRMLEKMGFCTYFLVVWDYIKYARDHGIPVGPGRGSAAGSLVAYCMKITNIDPVHHGLIFERFLNPERKSMPDVDTDFCIDRRDEMIEYVTQKYGEDKVAQIITFNRMTSKAVLKDVARVLDIPYAESDKMAKMIPVSRGKPAKLKVMISDQTPEPQFKARYDNEPWVKKWIDMAIRIEGTNKTFGVHAAGVVISSEPLDQIVPLQKNNDGAVITQYYMEDVEAMGLLKMDFLGLKNLTTLQRAVELVAETKGIELDLDDLPLQERKALQIRARTGSKKLPDDVKKTHKLLEAGDLEGIFQLESQGMKQIVRDLKPSGIEDISSILALYRPGPLDAGLIPIFINRKHGREEISYQHKLLEPILNETYGVLVYQEQIMKMAQDLADYSLGEADLLRRAMGKKKAEEMQKHRAKFVDGSTKHGVPSRIAENLFDQMVKFAEYCLSFGTEILTVEYGPLPIGKIVSEEINCSVYSVDPEGRVYTQAIAQWHDRGEQEVLEYELEDGSVIRATSDHRFLTTDYQLLAIEEIFARQLDLLTLENIKQTEEALDNHRLPFPLLDAGTIKMVKVIGRRSLGVQRIFDIGLPQDHNFLLANGAIAANCFNKSHSTAYAYVTYQTAYLKANYPVEYMAALLTASSDSQEKVEKYRENCQKMGITVEPPDINRSQRHFTPLGEAILFGLSAVRNLGEGAIEQIITARDNSEEKRFKSLADFCTQVDLRVVNRRAIETLIMAGAFDGVQSNRNQLLHDLELVIAWAQKRAKEKETGQLNIFDSLTAGESIKAKEAANNGFEQEPSAPPVAEFSLQEKLQLEKEHLGFYVSEHPLKSVQRSARLLSPINLVDLDQYKVRQKVSAVAILVAVKKIITKKNGQPMAFLTLEDMSGQSEAVVFPSNYERLQDVLIEGSQQMIWGKVDRRDDQYQLIVEDLEPVEEVKMVMLDLTPQEIANTSTQARLKQILQSHAPQKEQMKIPVVACINEGPTKHFIRFGENYWVNDYGTAVASLQELGFRARLEPLLPQSQSVG

External Links

PDB ID: 5OL1
EC: -
MEROPS: -
UniProt ID: -
NCBI: -

Substrate Specificity

P1'
Cys/Thr/Ser

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization
  • C-terminal thioester formation
  • Specificity stringency: Prime-low, Nonprime-low, only stringent to the P1"-Cys

Expression Platform:

  • E. coli

Mutations:

Other Properties:

-

References:

  1. [1] (Discovery, Expression) Shah, N. H.; Dann, G. P.; Vila-Perelló, M.; Liu, Z.; Muir, T. W. Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering. J Am Chem Soc 2012, 134 (28), 11338-11341. DOI: 10.1021/ja303226x