Catalyze the formation of an isopeptide bond between K31 on itself and D117 on SpyTag
Expression Platform:
E.coli
Mutations:
-
Other Properties:
-
References:
[1]
(Discovery) Zakeri, B.; Fierer, J. O.; Celik, E.; Chittock, E. C.; Schwarz-Linek, U.; Moy, V. T.; Howarth, M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proceedings of the National Academy of Sciences 2012, 109 (12), E690-E697.
DOI: 10.1073/pnas.1115485109
[2]
(Structure) Li, L.; Fierer, J. O.; Rapoport, T. A.; Howarth, M. Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag. Journal of Molecular Biology 2014, 426 (2), 309-317.
DOI: https://doi.org/10.1016/j.jmb.2013.10.021
[3]
(Application) Veggiani, G.; Nakamura, T.; Brenner, M. D.; Gayet, R. V.; Yan, J.; Robinson, C. V.; Howarth, M. Programmable polyproteams built using twin peptide superglues. Proceedings of the National Academy of Sciences 2016, 113 (5), 1202-1207.
DOI: 10.1073/pnas.1519214113