Catalyze the isopeptide bond formation between SpyTag and K-tag, the C- and N-terminal region of the original protein CnaB2, used in protein modification
Expression Platform:
E.coli
Mutations:
-
Other Properties:
-
References:
[1]
(Design, Application) Fierer, J. O.; Veggiani, G.; Howarth, M. SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Proc Natl Acad Sci U S A 2014, 111 (13), E1176-1181.
DOI: 10.1073/pnas.1315776111