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SpyCatcher002

Host Organism

Streptococcus pyogenes

Type

Other Ligase

Sequence

MSYYHHHHHHHDYDIPTTENLTFQGAMVTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATMELRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGEATKGDAHT

External Links

PDB ID: -
EC: -
MEROPS: -
UniProt ID: -
NCBI: -

Substrate Specificity

SpyTag002
VPTIVMV-D-AYKRYK

Functions

  • Catalyze the formation of an isopeptide bond between K31 on itself and D117 on SpyTag

Expression Platform:

  • Expi293

Mutations:

  • Rate improve >12-fold: D2T/S9G/Q13P/I19T/K37R/Q62H/K105E/I113T

Other Properties:

-

References:

  1. [1] (Engineering, Sequence, Activity) Keeble, A. H.; Turkki, P.; Stokes, S.; Khairil Anuar, I. N. A.; Rahikainen, R.; Hytonen, V. P.; Howarth, M. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A 2019, 116 (52), 26523-26533. DOI: 10.1073/pnas.1909653116