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VyPAL2

Host Organism

Viola yedoensis Makino (a synonym of Viola philippica var. philippica)

Type

PAL

Sequence

MQLFAAGVILFFLLALSGTIAGGLDVDSLQLPSEAAKFFHNDNSTNDDDSIGTRWAVLIAGSKGYHNYRHQADVCHMYQILRKGGVKDENIIVFMYDDIAYNESNPFPGIIINKPGGENVYKGVPKDYTGEDINNVNFLAAILGNKSAIIGGSGKVLDTSPNDHIFIYYADHGAPGKIGMPSKPYLYADDLVDTLKQKAATGTYKSMVFYVEACNAGSMFEGLLPEGTNIYAMAASNSTEGSWITYCPGTPDFPPEFDVCLGDLWSITFLEDCDAHNLRTETVHQQFELVKKKIAYASTVSQYGDIPISKDSLSVYMGTDPANDNRTFVDENSLRPPLKVIHQHDADLYHIWCKYNMAPEGSSKKIEAQKQLLELMSHRAHVDNSITLIGKLLFGVNKASKVLNTVRPVGQPLVDDWQCLKAMIRTFETHCGSLSEYGMKHTLSFANMCNAGIQKEQLAEAAAQACVTFPSNPYSSLAEGFSA

External Links

PDB ID: 6IDV
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: A0A509GV09
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I/F

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization, max. kcat/Km: 2.7 x 10^5 M-1s-1
  • Specificity strigency: Prime-low, Nonprime-high

Expression Platform:

  • Sf9-bacuolarvirus

Mutations:

-

Other Properties:

-

References:

  1. [1] Hemu, X.; El Sahili, A.; Hu, S.; Wong, K.; Chen, Y.; Wong, Y. H.; Zhang, X.; Serra, A.; Goh, B. C.; Darwis, D. A.; et al. Structural determinants for peptide-bond formation by asparaginyl ligases. Proc. Natl. Acad. Sci. U.S.A. 2019, 116 (24), 11737-11746. (Sequence, activity, structure) DOI: 10.1073/pnas.1818568116