Catalyze the isopeptide bond formation between SnoopTagJr and DogTag derived from the terminal regions of the original protein RrgA, used in protein conjugation and cyclizaiton
Expression Platform:
E.coli
Mutations:
Rate improve > 60-fold: A808P/Q837P/D838G/I839V
Other Properties:
-
References:
[1]
(Design, Sequence, Activity) Buldun, C. M.; Jean, J. X.; Bedford, M. R.; Howarth, M. SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation. J Am Chem Soc 2018, 140 (8), 3008-3018.
DOI: 10.1021/jacs.7b13237