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Aqualigase

Host Organism

Bacillus subtilis

Type

Subtiligase

Sequence

AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQDDLLHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGALFGTSMASAHVAGAAALILSKHPNWTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ

External Links

PDB ID: -
EC: 3.4.21.62
MEROPS: S8 subtilase
UniProt ID: P00782
NCBI: -

Substrate Specificity

P1
theoritically any residue

Functions

  • Intermolecular backbone terminal ligation
  • Amine−carboxyl dehydration condensation

Expression Platform:

  • Bacillus subtilis

Mutations:

  • Protease-to-ligase conversion: P225A/N62L/S63L/Y217L/N218F

Other Properties:

-

References:

  1. [1] (Engineering) Feng, Y.; Yan, X.; Ma, M.; Chen, R.; Zhang, C.; Cong, Y.; Fang, B.; Chen, C.; Dai, L.; Li, H.; et al. Aqualigase: A Star Enzyme for One-Step Peptide Bond Dehydration Condensation in a Nature Aqueous Phase. ACS Catalysis 2025, 11594-11607. DOI: 10.1021/acscatal.5c01532