Catalyze the isopeptide bond formation between DogCatcher Lys742 (K9) and DogTag Asn854 (N17) derived from the terminal regions of the original protein RrgA, used in protein conjugation and cyclizaiton
Expression Platform:
E.coli
Mutations:
Rate improve > 250-fold than SpyCatcher003
Other Properties:
-
References:
[1]
(Design, Sequence, Activity) Keeble, A. H.; Yadav, V. K.; Ferla, M. P.; Bauer, C. C.; Chuntharpursat-Bon, E.; Huang, J.; Bon, R. S.; Howarth, M. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol 2022, 29 (2), 339–350 e310.
DOI: 10.1016/j.chembiol.2021.07.005