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VdiPAL1

Host Organism

Viola dissecta

Type

PAL

Sequence

MKLLAAGAILFFLLALSGTVAVAGGLDVHPLRLPSEAAKFFHNDNSTSDDDSVGTRWAVLIAGSKGYNNYRHQADVCHMYQILRKGGVKDENIIVFMYDDIAYNESNPHPGILINKPGGEDVYKGVPKDYTGEDINNINFLAAILGNKSAIIGGSGKVLDTSPDDHIFIYYTDHGAPGKIGMPSKPYLYADDLVDTLKQKAAAGTYKSMVFYVEACNAGSMFEGLLPEGMNIYAMTASNSTEGSWVTYCPGTPDFPPEFDVCLGDLWSITFLEDCDAHNLRTETVHQQFELIKKKIAYASTVSQYGDIPISNYSLSVYMGTDPANDNRTFVDENSLRPPLKVLHQRDADLYHLWYKYQNTPEGSSKKIEAQKQLLELMTHRAHVDNSITLIGKLLFGMDNPSKMLNSVRPAGQPLVDDWQCLKTMIRTFETHCGSLSEYGMKHTLSFANMCNAGIRKEQLAEAAAQACVTFPSNSYSSLAEGFSA

External Links

PDB ID: 9VSX
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI: -

Substrate Specificity

P1
P1'
P2'
P1''
P2''
N/D
X
L/I/F
X except Pro
L/I/F/W/Y/M

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization, max. kcat/Km: 4.42 x 10^5 M-1s-1
  • Specificity stringency: Prime-low, Nonprime-high
  • pH optimal: pH 6-6.5

Expression Platform:

  • E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] (Discovery, Sequence, Activity, Substrate specificity, Mechanism) Du, W., Qi, S., Zhen, M., Liao, M., Zhao, Y., Ma, J., Hao, Y., Jiang, H., Lu, G., Liew, C. W., Chua, N., Chen, H., Hu, G., Hemu, X. (2025). Mining of natural diversity enables efficient and expressible peptide asparaginyl ligases, bioRxiv, DOI: 10.64898/2025.12.16.694575