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VyPAL2

Host Organism

Viola yedoensis Makino (a synonym of Viola philippica var. philippica)

Type

PAL

Sequence

MQLFAAGVILFFLLALSGTIAGGLDVDSLQLPSEAAKFFHNDNSTNDDDSIGTRWAVLIAGSKGYHNYRHQADVCHMYQILRKGGVKDENIIVFMYDDIAYNESNPFPGIIINKPGGENVYKGVPKDYTGEDINNVNFLAAILGNKSAIIGGSGKVLDTSPNDHIFIYYADHGAPGKIGMPSKPYLYADDLVDTLKQKAATGTYKSMVFYVEACNAGSMFEGLLPEGTNIYAMAASNSTEGSWITYCPGTPDFPPEFDVCLGDLWSITFLEDCDAHNLRTETVHQQFELVKKKIAYASTVSQYGDIPISKDSLSVYMGTDPANDNRTFVDENSLRPPLKVIHQHDADLYHIWCKYNMAPEGSSKKIEAQKQLLELMSHRAHVDNSITLIGKLLFGVNKASKVLNTVRPVGQPLVDDWQCLKAMIRTFETHCGSLSEYGMKHTLSFANMCNAGIQKEQLAEAAAQACVTFPSNPYSSLAEGFSA

External Links

PDB ID: 6IDV, 7f5P, 7F5Q, 7FA0
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: A0A509GV09
NCBI: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I/F

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization, max. kcat/Km: 2.7 x 10^5 M-1s-1
  • Specificity strigency: Prime-low, Nonprime-high

Expression Platform:

  • Sf9-bacuolarvirus

Mutations:

Other Properties:

-

References:

  1. [1] (Sequence, activity, structure) Hemu, X.; El Sahili, A.; Hu, S.; Wong, K.; Chen, Y.; Wong, Y. H.; Zhang, X.; Serra, A.; Goh, B. C.; Darwis, D. A.; et al. Structural determinants for peptide-bond formation by asparaginyl ligases. Proc. Natl. Acad. Sci. U.S.A. 2019, 116 (24), 11737-11746. DOI: 10.1073/pnas.1818568116
  2. [2] (Substrate-specificity extension) Li, H.; Xia, Y.; Balamkundu, S.; Liu, J.; Li, F.; Zhang, X.; Lescar, J.; Tam, J. P.; Preiser, P. R.; Liu, C.-F. Peptidyl Asparaginyl Ligase-Mediated Orthogonal Ligation at Non-Asx Peptide Bonds for d-Peptide Cyclization and Antibody Dual Labeling. J. Am. Chem. Soc. 2026, 148 (1), 1989–1997. DOI: 10.1021/jacs.5c20426