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VyPAL2-I244V

Host Organism

Viola yedoensis Makino (a synonym of Viola philippica var. philippica)

Type

PAL

Sequence

MQLFAAGVILFFLLALSGTIAGGLDVDSLQLPSEAAKFFHNDNSTNDDDSIGTRWAVLIAGSKGYHNYRHQADVCHMYQILRKGGVKDENIIVFMYDDIAYNESNPFPGIIINKPGGENVYKGVPKDYTGEDINNVNFLAAILGNKSAIIGGSGKVLDTSPNDHIFIYYADHGAPGKIGMPSKPYLYADDLVDTLKQKAATGTYKSMVFYVEACNAGSMFEGLLPEGTNIYAMAASNSTEGSWVTYCPGTPDFPPEFDVCLGDLWSITFLEDCDAHNLRTETVHQQFELVKKKIAYASTVSQYGDIPISKDSLSVYMGTDPANDNRTFVDENSLRPPLKVIHQHDADLYHIWCKYNMAPEGSSKKIEAQKQLLELMSHRAHVDNSITLIGKLLFGVNKASKVLNTVRPVGQPLVDDWQCLKAMIRTFETHCGSLSEYGMKHTLSFANMCNAGIQKEQLAEAAAQACVTFPSNPYSSLAEGFSA

External Links

PDB ID: 7F5J
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I/F

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization, max. kcat/Km: 2.7 x 10^5 M-1s-1
  • Specificity strigency: Prime-low, Nonprime-high

Expression Platform:

  • Sf9-bacuolarvirus

Mutations:

  • Fast kinetics: I244V

Other Properties:

-

References:

  1. [1] (Activation mechanism, engineering) Hu, S.; El Sahili, A.; Kishore, S.; Wong, Y. H.; Hemu, X.; Goh, B. C.; Sang, Z.; Wang, Z.; Tam, J. P.; Liu, C.-F.; et al. Structural basis for proenzyme maturation, substrate recognition, and ligation by a hyperactive peptide asparaginyl ligase. Plant Cell 2022, koac281. DOI: 10.1093/plcell/koac281