VaPAL2 - Peptide Ligase Database

Host Organism

Viola albida Palib.

Type

PAL

Sequence

MKLLAAGVILFFLLALSGTVAGGLDVDPLRLPSEAAKFFHNDNSTSDDDSVGTRWAVLIAGSKGYNNYRHQADVCHMYQILRKGGVKDENIIVFMYDDIAYNESNPHPGILINKPGGEDVYKGVPKDYTGEDINNINFLAAILGNKSAIIGGSGKVLDTSPNDHIFIYYADHGAPGKIGMPSKPYLYADDLVDTLKQKAATGTYKSMVFYVEACNAGSMFEGLLPEGMNIYAMTASNSTEGSLITYCAGVTPGVPLEIVTCLGDLWSITFLEDCDAHNLRTETVHQQFELVKKRIAYASTVSQYGDIPISKDSLSVYMGTDPANDNRTFVDENSLRPPLKVIHQRDADLYHLWYKYQNIPEGSSKKIEAQKQLLELMSHRAHVDNSITLIGKLLFGVDKASKVLNTVRPVGQPLVDDWQCLKAMIRTFETHCGSLSEYGMKHTLSFANMCNAGIQKEQLDEAAAQACVTFPSNSYSSLAEGFSA

External Links

PDB ID: -

EC: 3.4.22.34

MEROPS: C13 legumain

UniProt ID: -

NCBI accession: -

Substrate Specificity

P1'

P2'

N/D

L/I/F

Functions

Backbone terminal cyclization
Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

E. coli

Mutations:

Other Properties:

References:

[1] Hemu, X.; Chan, N. Y.; Liew, H. T.; Hu, S.; Zhang, X.; Serra, A.; Lescar, J.; Liu, C. F.; Tam, J. P. Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains. New Phytol. 2023, 238 (4), 1534-1545. (Sequence, activity) DOI: 10.1111/nph.18841